![]() ![]() The melting temperature (Tm) values of Fov isolates were in the range 86.83 to 87.02. The sensitivity of real-time PCR allowed detection of 200 fg of target genomic DNA. PCR product of the expected size was amplified from as litde as 100 fg of purified Fov genomic DNA. sambucinum, Rhizoctonia solani, and Macrophomina phaseolina. ![]() By using the primer pair Fovl-Eg-f and Fovl-Eg-r, a 438-bp DNA fragment was consistently amplified from 41 Egyptian isolates of Fov (race 3), while no amplification was obtained from template genomic DNAs obtained from other fungal pathogens, namely F. vasinfectum (Fov), a fungus causing Fusarium wilt of cotton, in infected cotton seedlings. A PCR assay based on a pair of oligonucleotide primers targeting the 16S and 23S rRNA genes was used to detect Fusarium oxysporum f.
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